Gene Expression

Introduction

Gene expression
The phenotypic manifestation of a gene or genes by the processes of genetic transcription and genetic translation.
Gene expression analysis (profiling)
The determination of the pattern of genes expressed at the level of genetic transcription, under specific circumstances or in a specific cell.

Gene expression analysis is used to study regulatory gene defects in cancer and other devastating diseases, cellular responses to the environment, cell cycle variation, etc.

How It Works

When genes are expressed, the genetic information (base sequence) on DNA is first transcribed (copied) to a molecule of messenger RNA (mRNA). The mRNA molecules then leave the cell nucleus and enter the cytoplasm, where they participate in protein synthesis by specifying the particular amino acids that make up individual proteins.

Technologies

• Real Time quantitative RT-PCR
  • The Applied Biosystems TaqMan^® Gene Expression Assays
  • The Invitrogen D-LUX™ Gene Expression Assays
• In situ hybridization by using riboprobes (GENSAT project)
• Microarrays
  • Affymetrix GeneChip^® expression arrays
• Serial Analysis of Gene Expression (SAGE)
• Massively Parallel Signature Sequencing (MPSS)

Probe

Resources

Disclaimer

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