Perlegen Sciences, Inc.

About

Perlegen Sciences closed operations on Oct. 30, 2009.

Perlegen was founded in 2000 with the mission of identifying patterns of genetic variation between individuals and using this information to improve patient care and uncover the genetic foundations of deseases.

Perlegen actively participated in International HapMap Project.

Perlegen Genotyping Protocol (HapMap)

Light-directed chemical synthesis of oligonucleotide arrays was carried out by Affymetrix, Inc. (Santa Clara, CA). Genomic DNA was amplified by multiplex long-range PCR, then all PCR products destined for a single genotyping array were pooled together and end-labeled with biotinylated nucleotides. Each labeled DNA sample was hybridized to a chip. The hybridized chips were washed, then incubated with streptavidin, washed and incubated with biotinylated anti-streptavidin antibody followed by another washing. Then, chips were stained with streptavidin-Cy-chrome conjugate followed by washing. Hybridization of the sample to the array was detected using a confocal laser scanner.

Individual genotypes for a SNP were determined by clustering measurements from multiple scans in the two-dimensional space defined by background-adjusted intensities of the perfect-match features for the reference and alternate alleles.

Probe

References

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